Northwestern University NU Office for Research Shared and Core Facilities




The mission of the Northwestern University Mouse Histology & Phenotyping Laboratory (MHPL) is to assist primary investigators and researchers with standard and customized research-specific histology services. The laboratory provides comprehensive histology services for a wide variety of tissues (i.e., mouse, rat, sheep, pig, zebrafish, etc.) for routine histology, immunohistochemistry, whole mouse/rat necropsy, and phenotyping of organs and tissues. Pathologist consultation can also be provided to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding the biologic actions of the targeted molecule(s) or the toxicity of exogenously administered substances. In addition, the laboratory provides training opportunities for learning histology techniques and phenotyping analysis.

Services Offered

    • Necropsy and tissue collection
    • Gross exam with report
    • Histopathology (slide interpretation)
    • Histopathology toxicity evaluation
    • Photo documentation of histopathology
    • Training in necropsy techniques including perfusion of mice and special tissue dissection
    • Phenotype screening (complete necropsy and histopathology of mutant mice)
    • Assistance with development of animal pathology protocols
    • Immunohistochemistry (IHC) – Using tyramide signal amplification (TSA) and ABC methods (automated), with DAB chromogenic substrate. See documents below for details.
    • Immunofluorescence (IF)- Single and multiple staining
    • TUNEL assay
    • Freezing and embedding of tissues for histology
    • Frozen sections
    • Paraffin block processing and Paraffin sections
    • Hematoxylin and Eosin staining of sections
    • Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains)








Northwestern University

Research Home  |  Research Administrative Offices  |

Office for Research - 633 Clark Street, Evanston, IL 60208-1108

The Office for Research promotes, facilitates and enhances research at Northwestern University.

© 2007-2011 Northwestern University

Northwestern Home | World Wide Web Disclaimer | University Policy Statements