Histology

Mouse Histology & Phenotyping Laboratory Services include:

  • Paraffin block processing and Paraffin sections
  • Hematoxylin and Eosin staining
  • Specialized histochemical staining
  • Freezing and embedding of tissues for histology
  • Frozen sections
  • Consultation

Download PDF – List of Special Staining 4-22-2016

List of Special Staining

NameViewClassificationLevel
Alcian Blue pH1sulfated mucosubstances2
Alcian Blue pH 2.5Viewacid mucopolysaccharides2
Bielchowskynerve fibers, neurofibrillary tangles, senile plaques3
Brown and Brenn (Modified)gram positive and gram negative bacteria2
Cresyl VioletNissl body cytoplasm of Neurons1
Fontana Massonargentaffin (melanin) granules2
Gordon and Sweet’s method – for reticulinreticular fibers3
Grocott’s Methanamine Silver Method (GMS)fungi, pneumocystis carnii, histoplasma SPP3
Hall’s Bilirubin stainBile Stain2
Jones Methanamine Silver MethodViewbasement membranes3
Luxol Fast Bluemyelin3
Luxol Fast Blue – Cresyl VioletViewmyelin & Nissl Substance3
Mucicarmine (Mayer’s Method)Viewacidic mucins, sulphated mucins. Cryptococcus neoformans2
Muller-mowry colloidal ironCarboxylated, sulfated mucopolysaccharides,glycoproteins2
Nuclear Fast Red (Kernechtrot)counterstain1
Oil Red Olipids1
PAS with Diastase Digestionglycogen2
Periodic Acid Schiff (PAS)Viewpolysaccharides, basement membranes, mucosubstances, glycogen2
Picro Sirius RedDistinguishes collagen I from collagen III2
Prussian Blue Reactioniron- Ferric Form1
Toluidine blue Acidifiedmast cells1
Trichrome- Gomoris One-Stepcollagen and smooth muscle
2
2
Trichrome - Masson'scollagen and smooth muscle2
Verhoff-Van Geison (VVG)elastic fibers2
Von Kossacalcium deposits/salts3
Weigert’s Resorcin Fuchsinelastic fibers2
Zeil-Neelsen Acid-fast Stainbacteria2
Herovici staincollagen2
Congo redamyloid2
Fite Acid-fast StainLoprosy Organisms2
Thioflavin stainamyloid1
Steiner and SteinerH pylori/Causative organism of legionellosis3

 

*Legend
Level-1$7.87 RHLCC member
Level-2$10.6 RHLCC member
Level-3$16.27 RHLCC member