IHC, IF and TUNEL

The Mouse Histology & Phenotyping Laboratory (MHPL) offers extensive immunohistochemistry (IHC), Immunofluorescence (IF) and TUNEL services that provide consistant and standardized protocols on large and small scale projects on formalin fixed, paraffin embedded (FFPE) or frozen tissues. Sections to be stained (paraffin or frozen) should be brought to the MHPL, or they can be generated by the MHPL. Antibodies can be provided by the user or MHPL. Please contact us for an updated list of antibodies. The MHPL also provides antibody “workup” services to test novel or untried antibodies on your tissue of interest. Please contact us to arrange a consultation.                                                                                         

  Services include

  • Immunohistochemistry Staining
  • Immunofluorescence – Single and Multiple
  • TUNEL assay
  • Custom antibody work-up
  • Consultation

IHC Submission Instructions

Download PDF: Primary Antibody List 1-10-2017

Download PDF: Primary Antibodies Work-up List 1-10-2017

 

 

Primary Antibody List 1-10-2017

Primary AntibodyClassificationReactivityHost#
Ki67 (TEC-3)ProliferationmRat-mAb1
Glial Fibrillary Acidic Protein (GFAP)Astrocyteh.m.rtRabbit-p4
S100 (1A4)Neural differentiationh.m.rtRabbit-p5
SMA (alpha)(C04018)Smooth Muscle Actinh.m.rtRabbit-mAb7
MITF(C5)Melanocyte/Melanomah.rtMs-mAb9
Melanoma CocktailMelanomah.rtMs-mAb12
CD31/PECAM-1 (Mec13.3)Endothelial cellmRat-mAb13
Digoxigenin--Sheep-p14
Progesterone Receptor (PR)Steroid receptorh.m.rtRabbit-p15
Glucagon (EP3070)Hormoneh.m.rtRabbit-mAb16
Desmin (Y266)Smooth, skeletal muscle differentiationh.m.rtRabbit-mAb17
Vimentin (EPR3776)Intermediate filamenth.m.rtRabbit-mAb18
Histone H3 (phospho S10)Proliferationh.m.rtRabbit-p19
CD34 (MEC14.7)Endothelial cellmRat-mAb21
Cyclin D1Proliferationh.m.rtRabbit-p22
Caspase 9 (aa 1-134)Apoptosish.m.rtRabbit-p23
Glucose-Regulated Protein 94 (Grp94/gp96)Endoplasmic reticulumh.m.rtRat-mAb24
CD45R/B220 (RA3-6B2)B cellmRat-mAb25
Mac 3 (M3/84)MacrophagemRat-mAb26
p27/Kip1 (EPFHCR16)Differentiationm.rtRabbit-mAb27
F4/80 (BM8)Macrophage markermRat-mAb28
Cadherin, Pan Plasma Membraneh.m.rtRabbit-p29
NeuN (A60)Neuronalh.rtMs-mAb30
CD138 (281-2)Plasma cellmRat-mAb31
Casp3 cleaved/activatedApoptosish.m.rtRabbit-p32
Von Willebrand Factor (vWF)Endothelial cellm.rtRabbit-p33
Cyclooxygenase 2 (COX2)Inflammatory responseh.m.rtRabbit-mAb34
Estrogen Receptor (alpha-ER) (E115)Steroid receptorh.m.rtRabbit-mAb35
Protein Gene Product 9.5 (PGP9.5)Neuronalh.m.rtRabbit-p36
CD34Endothelial cellh.m.rt.rbRabbit-p37
CD45 (30-F11) (Fronze only)Leukocyte common antigenmRat-mAb38
Ki67 (SP6)Proliferationh.m.rtRabbit-mAb39
HER2/ErbB2 (29D8)Oncogeneh.m.rtRabbit-mAb40
Desmoglein 1Component of intercellular desmosome junctionsh.m.rtRabbit-mAb41
Galactosidase (Beta-gal)Hydrolase enzyme-Rabbit-p43
Androgen ReceptorSteroid receptorh.m.rtRabbit-p44
CD31/PECAM-1Endothelial cellh.m.rtGoat-p45
Cytokeratin-10Epithelial differentiationh.mRabbit-p46
TTF-1 (8G7G3/1)Differentiation lung-cancerh.rtMs-mAb47
CD45 (PD7/26/16 + 2B11)Leukocyte common antigenh.rtMs-mAb49
Ki67 (8D5)Proliferationh.m.rt.rb.pMs-mAb50
p44/42 MAPK (Erk1/2) (phospho Thr202/Tyr204)Signal transducth.m.rtRabbit-mAb51
CREB (48H2)Signal transducth.m.rtRabbit-mAb53
CREB (phospho Ser133)Signal transducth.m.rtRabbit-mAb54
S6 Ribosomal Protein (phospho Ser235/236)Signal transducth.m.rtRabbit-mAb55
EGFR (E114)Epithelial differentiationh.m.rtRabbit-mAb56
p38 MAPK (phospho Thr180/Tyr182)Signal transducth.m.rtRabbit-mAb57
Akt (phospho Ser473)Signal transducth.m.rtRabbit-mAb58
Peanut Agglutinin (PNA-Biotinylated) *--Lectin59
CD68Macrophagem,rtRabbit-p60
SMA (alpha) (1A4)Smooth Muscle ActinrbMs-mAb61
PCNAProliferationh.m.rtRabbit-p62
TTF-1 (EPR5955(2)Differentiation lung-cancerh.m.rtRabbit-mAb63
Fas LTumor necrosis factor familyh.m.rtRabbit-p65
Ym1MacrophagemGoat-p66
CD24B-cellsh.m.rtRabbit-p68
Akt1 (C73H10)Signal transducth.m.rtRabbit-mAb70
Stat3aSignal transducth.m.rtRabbit-mAb71
Stat3 (phospho Tyr705)Signal transducth.m.rtRabbit-mAb72
IgG (HRP)Antibody isotypemGoat-p73
RFPRed fluorescent proteinRFPRabbit-p75
ATP1a3Na/K ATPasem.rtRabbit-p78
proSP-CType II alveolar epithelial cellsh.m.rtRabbit-p79
CD8a (Fronze only)T cellmRat-mAb81
BrduProliferation-Sheep-p82
Pax-7Myogenesish.m.rtRabbit-p83
HIF-1aEndogenous hypoxia markersh.mRabbit-p84
Cadherin, N-Metastatic carcinoma unknown primarymRat-mAb85
Cytokeratin 19Epithelial tumor markermRat-mAb87
EpCAM (G8.8)Carcinoma markermRat-mAb88
BrduProliferation-Ms-mAb90
GFPGreen fluorescent protein-Ms-mAb91
InsulinPancreatic endocrine tumorsh.m.rtGuinea Pig-p94
Collagen IFibrillar-forming-collagenh.mGoat-p95
Ly-6G (1A8)GranulocytesmRat-mAb96
Gr-1 (Biotin) (RB6-8C5)(Fronze only)Monocyte, neutrophils and eosinophils (granulocytes)mRat-mAb97
Lam-a1Basement membraneh.mRabbit-p98
LamininBasement membraneh.m.rtChicken-p99
Galactosidase (Beta-gal) (IF)Hydrolase enzyme-Chicken-p100
TransferrinErythoid markerh.m.rtRabbit -p102
GFP (Biotin)Green fluorescent protein-Goat-p103
c-Myc (Y69)Proto-oncogene/Proliferationh.m.rtRabbit-mAb104
LC3A (D50G8)Autophagosome Markerh.m.rtRabbit-mAb107
CD3 (SP7)T cellh.m.rtRabbit-mAb108
CD43 (S7)Cell adhesion and T-cell activationmRat-mAb109
CD44 (IM7)Cell adhesion and migrationmRat-mAb110
TER-119/Erythroid CellsErythroid Cell MarkermRat-mAb111
Ki67 (B56)Proliferationh.m.rt.pMs-mAb112
CD11c (HL3)(Fronze only)Dendritic cellsm.Hamster-mAb113
VEGFMisc tumorsh.m.rtPabbit-p114
Smad2 (phospho Ser465/467)Signal transducth.m.rtRabbit-p117
CD68 (KP1)MacrophagehMs-mAb122
CDk-2Cell cycle markerh.m.rtRabbit-p125
EMBPEosinophil markerh.m.rtGoat-p126
Hypophosphorylated Neurofilament H (NF-200)Neurodegeneration markerh.m.rMs-mAb128
Tyrosine HydroxylaseDopaminergic neuron markerh.mRabbit-p129
SMA Ab-1 (alpha) (1A4) (Biotin)Smooth Muscle Actinh.m.rt,rbMs-mAb130
FSP1Fibroblast markerh.mRabbit-p131
PDGFæR alphaPlatelet Derived Growth Factor ReceptmGoat-p132
GFPGreen fluorescent protein-Chichen IgY133
CD11c (N418)(Fronze only)Dendritic cellsh.mHamster-mAb135
p53(FL-393)tumor suppressor geneh.m.rRabbit-p137
E-Cadherin (36/E-Cadherin) Cell-cell interaction, Differentiationh.m.rMs-mAb140
N-Cadherin (32/N-Cadherin)Metastatic carcinoma unknown primaryh.m.rMs-mAb141
Cyclin D1 / Bcl-1 Antibody (SP4)Proliferationh.m.rRabbit-mAb142
CD45Leukocyte differentiationh.m.r.pRabbit-p143
CD20B-cellhRabbit-mAb144
PAX5Transcription Factor (B cell)h.m.rRabbit-p145
Cyclin B1 AB-2Proliferationh.m.rRabbit-p146
CD31 Zinc-fixationEndothelial cellsm.Rat-mAb147
CD8 Zinc-fixationT cellh.m.rtRabbit-mAb148
Beta-Tubulin (9F3)Neuronal Markerh.m.rtRabbit-mAb149
Cyclin A AB-7Proliferationh.m.rRabbit-p150
FOXP3Transcription regulator of T Cellsh.m.rRabbit-p151
Fra-2 (H-103)Transcription Factorh.m.rRabbit-p152
CD56 (RNL-1)Neuroendocrine differentiationh.m.rMs-mAb155
Caspase-3 (3CSP03)Apoptosish.rbMs-mAb156
Beta III TubulinNeuronal Markerh.m.r.pRabbit-p157
VCAM1[EPR5047]Activated Endothelial Cellh.m.rtRabbit-mAb158