IHC, IF and TUNEL

The Mouse Histology & Phenotyping Laboratory (MHPL) offers extensive immunohistochemistry (IHC), Immunofluorescence (IF) and TUNEL services that provide consistant and standardized protocols on large and small scale projects on formalin fixed, paraffin embedded (FFPE) or frozen tissues. Sections to be stained (paraffin or frozen) should be brought to the MHPL, or they can be generated by the MHPL. Antibodies can be provided by the user or MHPL. Please contact us for an updated list of antibodies. The MHPL also provides antibody “workup” services to test novel or untried antibodies on your tissue of interest. Please contact us to arrange a consultation.                                                                                         

  Services include

  • Immunohistochemistry Staining
  • Immunofluorescence – Single and Multiple
  • TUNEL assay
  • Custom antibody work-up
  • Consultation

IHC Submission Instructions

Download PDF: Primary Antibody List 6-1-2016 

Download PDF: Primary Antibodies Work-up List 5-2-2016

 

 

Primary Antibody List 6-1-2016

Primary AntibodyClassificationReactivityHost#
Akt (phospho Ser473)Signal transducth.m.rtRabbit-mAb58
Akt1 (C73H10)Signal transducth.m.rtRabbit-mAb70
Androgen ReceptorSteroid receptorh.m.rtRabbit-p44
ATP1a3Na/K ATPasem.rtRabbit-p78
BrduProliferation-Sheep-p82
BrduProliferation-Ms-mAb90
Cadherin, N-Metastatic carcinoma unknown primarymRat-mAb85
Cadherin, Pan Plasma Membraneh.m.rtRabbit-p29
Casp3 cleaved/activatedApoptosish.m.rtRabbit-p32
Caspase 9 (aa 1-134)Apoptosish.m.rtRabbit-p23
CD11c (HL3)(Fronze only)Dendritic cellsm.Hamster-mAb113
CD11c (N418)(Fronze only)Dendritic cellsh.mHamster-mAb135
CD138 (281-2)Plasma cellmRat-mAb31
CD24B-cellsh.m.rtRabbit-p68
CD3 (SP7)T cellh.m.rtRabbit-mAb108
CD31/PECAM-1Endothelial cellh.m.rtGoat-p45
CD31/PECAM-1 (Mec13.3)Endothelial cellmRat-mAb13
CD34Endothelial cellh.m.rt.rbRabbit-p37
CD34 (MEC14.7)Endothelial cellmRat-mAb21
CD43 (S7)Cell adhesion and T-cell activationmRat-mAb109
CD44 (IM7)Cell adhesion and migrationmRat-mAb110
CD45 (30-F11) (Fronze only)Leukocyte common antigenmRat-mAb38
CD45 (PD7/26/16 + 2B11)Leukocyte common antigenh.rtMs-mAb49
CD45R/B220 (RA3-6B2)B cellmRat-mAb25
CD68Macrophagem,rtRabbit-p60
CD68 (KP1)MacrophagehMs-mAb122
CD8a (Fronze only)T cellmRat-mAb81
CDk-2Cell cycle markerh.m.rtRabbit-p125
c-Myc (Y69)Proto-oncogene/Proliferationh.m.rtRabbit-mAb104
Collagen IFibrillar-forming-collagenh.mGoat-p95
CREB (48H2)Signal transducth.m.rtRabbit-mAb53
CREB (phospho Ser133)Signal transducth.m.rtRabbit-mAb54
Cyclin D1Proliferationh.m.rtRabbit-p22
Cyclooxygenase 2 (COX2)Inflammatory responseh.m.rtRabbit-mAb34
Cytokeratin 19Epithelial tumor markermRat-mAb87
Cytokeratin-10Epithelial differentiationh.mRabbit-p46
Desmin (Y266)Smooth, skeletal muscle differentiationh.m.rtRabbit-mAb17
Desmoglein 1Component of intercellular desmosome junctionsh.m.rtRabbit-mAb41
Digoxigenin--Sheep-p14
EGFR (E114)Epithelial differentiationh.m.rtRabbit-mAb56
EMBPEosinophil markerh.m.rtGoat-p126
EpCAM (G8.8)Carcinoma markermRat-mAb88
Estrogen Receptor (alpha-ER) (E115)Steroid receptorh.m.rtRabbit-mAb35
F4/80 (BM8)Macrophage markermRat-mAb28
Fas LTumor necrosis factor familyh.m.rtRabbit-p65
FSP1Fibroblast markerh.mRabbit-p131
Galactosidase (Beta-gal)Hydrolase enzyme-Rabbit-p43
Galactosidase (Beta-gal) (IF)Hydrolase enzyme-Chicken-p100
GFPGreen fluorescent protein-Ms-mAb91
GFP (Biotin)Green fluorescent protein-Goat-p103
Glial Fibrillary Acidic Protein (GFAP)Astrocyteh.m.rtRabbit-p4
Glucagon (EP3070)Hormoneh.m.rtRabbit-mAb16
Glucose-Regulated Protein 94 (Grp94/gp96)Endoplasmic reticulumh.m.rtRat-mAb24
Gr-1 (Biotin) (RB6-8C5)(Fronze only)Monocyte, neutrophils and eosinophils (granulocytes)mRat-mAb97
HER2/ErbB2 (29D8)Oncogeneh.m.rtRabbit-mAb40
HIF-1aEndogenous hypoxia markersh.mRabbit-p84
Histone H3 (phospho S10)Proliferationh.m.rtRabbit-p19
Hypophosphorylated Neurofilament H (NF-200)Neurodegeneration markerh.m.rMs-mAb128
IgG (HRP)Antibody isotypemGoat-p73
InsulinPancreatic endocrine tumorsh.m.rtGuinea Pig-p94
Ki67 (8D5)Proliferationh.m.rt.rb.pMs-mAb50
Ki67 (B56)Proliferationh.m.rt.pMs-mAb112
Ki67 (SP6)Proliferationh.m.rtRabbit-mAb39
Ki67 (TEC-3)ProliferationmRat-mAb1
Lam-a1Basement membraneh.mRabbit-p98
LamininBasement membraneh.m.rtChicken-p99
LC3A (D50G8)Autophagosome Markerh.m.rtRabbit-mAb107
Ly-6G (1A8)GranulocytesmRat-mAb96
Mac 3 (M3/84)MacrophagemRat-mAb26
Melanoma CocktailMelanomah.rtMs-mAb12
MITF(C5)Melanocyte/Melanomah.rtMs-mAb9
NeuN (A60)Neuronalh.rtMs-mAb30
p27/Kip1 (EPFHCR16)Differentiationm.rtRabbit-mAb27
p38 MAPK (phospho Thr180/Tyr182)Signal transducth.m.rtRabbit-mAb57
p44/42 MAPK (Erk1/2) (phospho Thr202/Tyr204)Signal transducth.m.rtRabbit-mAb51
Pax-7Myogenesish.m.rtRabbit-p83
PCNAProliferationh.m.rtRabbit-p62
PDGF R alphaPlatelet Derived Growth Factor ReceptmGoat-p132
Peanut Agglutinin (PNA-Biotinylated) *--Lectin59
Progesterone Receptor (PR)Steroid receptorh.m.rtRabbit-p15
proSP-CType II alveolar epithelial cellsh.m.rtRabbit-p79
Protein Gene Product 9.5 (PGP9.5)Neuronalh.m.rtRabbit-p36
RFPRed fluorescent proteinRFPRabbit-p75
S100 (1A4)Neural differentiationh.m.rtRabbit-p5
S6 Ribosomal Protein (phospho Ser235/236)Signal transducth.m.rtRabbit-mAb55
SMA (alpha) (1A4)Smooth Muscle ActinrbMs-mAb61
SMA (alpha)(C04018)Smooth Muscle Actinh.m.rtRabbit-mAb7
SMA Ab-1 (alpha) (1A4) (Biotin)Smooth Muscle Actinh.m.rt,rbMs-mAb130
Smad2 (phospho Ser465/467)Signal transducth.m.rtRabbit-p117
Stat3 (phospho Tyr705)Signal transducth.m.rtRabbit-mAb72
Stat3aSignal transducth.m.rtRabbit-mAb71
TER-119/Erythroid CellsErythroid Cell MarkermRat-mAb111
TransferrinErythoid markerh.m.rtRabbit -p102
TTF-1 (8G7G3/1)Differentiation lung-cancerh.rtMs-mAb47
TTF-1 (EPR5955(2)Differentiation lung-cancerh.m.rtRabbit-mAb63
Tyrosine HydroxylaseDopaminergic neuron markerh.mRabbit-p129
VEGFMisc tumorsh.m.rtPabbit-p114
Vimentin (EPR3776)Intermediate filamenth.m.rtRabbit-mAb18
Von Willebrand Factor (vWF)Endothelial cellm.rtRabbit-p33
Ym1MacrophagemGoat-p66
GFPGreen fluorescent protein-Chichen IgY133
p53(FL-393)tumor suppressor geneh.m.rRabbit-p137
E-Cadherin (36/E-Cadherin) Cell-cell interaction, Differentiationh.m.rMs-mAb140
N-Cadherin (32/N-Cadherin)Metastatic carcinoma unknown primaryh.m.rMs-mAb141
Cyclin D1 / Bcl-1 Antibody (SP4)Proliferationh.m.rRabbit-mAb142
* Lectin binding proteins
Abbreviation: h:human; m: mouse; rt:rat; rb: rabbit p: pig;