The Mouse Histology & Phenotyping Laboratory (MHPL) offers extensive immunohistochemistry (IHC), Immunofluorescence (IF) and TUNEL services that provide consistant and standardized protocols on large and small scale projects on formalin fixed, paraffin embedded (FFPE) or frozen tissues. Sections to be stained (paraffin or frozen) should be brought to the MHPL, or they can be generated by the MHPL. Antibodies can be provided by the user or MHPL. Please contact us for an updated list of antibodies. The MHPL also provides antibody “workup” services to test novel or untried antibodies on your tissue of interest. Please contact us to arrange a consultation.
Services include
- Immunohistochemistry Staining
- Immunofluorescence – Single and Multiple
- TUNEL assay
- Custom antibody work-up
- Consultation
Download PDF: Primary Antibody List 1-10-2017
Download PDF: Primary Antibodies Work-up List 1-10-2017
Primary Antibody List 1-10-2017
Primary Antibody Classification Reactivity Host #
Ki67 (TEC-3) Proliferation m Rat-mAb 1
Glial Fibrillary Acidic Protein (GFAP) Astrocyte h.m.rt Rabbit-p 4
S100 (1A4) Neural differentiation h.m.rt Rabbit-p 5
SMA (alpha)(C04018) Smooth Muscle Actin h.m.rt Rabbit-mAb 7
MITF(C5) Melanocyte/Melanoma h.rt Ms-mAb 9
Melanoma Cocktail Melanoma h.rt Ms-mAb 12
CD31/PECAM-1 (Mec13.3) Endothelial cell m Rat-mAb 13
Digoxigenin - - Sheep-p 14
Progesterone Receptor (PR) Steroid receptor h.m.rt Rabbit-p 15
Glucagon (EP3070) Hormone h.m.rt Rabbit-mAb 16
Desmin (Y266) Smooth, skeletal muscle differentiation h.m.rt Rabbit-mAb 17
Vimentin (EPR3776) Intermediate filament h.m.rt Rabbit-mAb 18
Histone H3 (phospho S10) Proliferation h.m.rt Rabbit-p 19
CD34 (MEC14.7) Endothelial cell m Rat-mAb 21
Cyclin D1 Proliferation h.m.rt Rabbit-p 22
Caspase 9 (aa 1-134) Apoptosis h.m.rt Rabbit-p 23
Glucose-Regulated Protein 94 (Grp94/gp96) Endoplasmic reticulum h.m.rt Rat-mAb 24
CD45R/B220 (RA3-6B2) B cell m Rat-mAb 25
Mac 3 (M3/84) Macrophage m Rat-mAb 26
p27/Kip1 (EPFHCR16) Differentiation m.rt Rabbit-mAb 27
F4/80 (BM8) Macrophage marker m Rat-mAb 28
Cadherin, Pan Plasma Membrane h.m.rt Rabbit-p 29
NeuN (A60) Neuronal h.rt Ms-mAb 30
CD138 (281-2) Plasma cell m Rat-mAb 31
Casp3 cleaved/activated Apoptosis h.m.rt Rabbit-p 32
Von Willebrand Factor (vWF) Endothelial cell m.rt Rabbit-p 33
Cyclooxygenase 2 (COX2) Inflammatory response h.m.rt Rabbit-mAb 34
Estrogen Receptor (alpha-ER) (E115) Steroid receptor h.m.rt Rabbit-mAb 35
Protein Gene Product 9.5 (PGP9.5) Neuronal h.m.rt Rabbit-p 36
CD34 Endothelial cell h.m.rt.rb Rabbit-p 37
CD45 (30-F11) (Fronze only) Leukocyte common antigen m Rat-mAb 38
Ki67 (SP6) Proliferation h.m.rt Rabbit-mAb 39
HER2/ErbB2 (29D8) Oncogene h.m.rt Rabbit-mAb 40
Desmoglein 1 Component of intercellular desmosome junctions h.m.rt Rabbit-mAb 41
Galactosidase (Beta-gal) Hydrolase enzyme - Rabbit-p 43
Androgen Receptor Steroid receptor h.m.rt Rabbit-p 44
CD31/PECAM-1 Endothelial cell h.m.rt Goat-p 45
Cytokeratin-10 Epithelial differentiation h.m Rabbit-p 46
TTF-1 (8G7G3/1) Differentiation lung-cancer h.rt Ms-mAb 47
CD45 (PD7/26/16 + 2B11) Leukocyte common antigen h.rt Ms-mAb 49
Ki67 (8D5) Proliferation h.m.rt.rb.p Ms-mAb 50
p44/42 MAPK (Erk1/2) (phospho Thr202/Tyr204) Signal transduct h.m.rt Rabbit-mAb 51
CREB (48H2) Signal transduct h.m.rt Rabbit-mAb 53
CREB (phospho Ser133) Signal transduct h.m.rt Rabbit-mAb 54
S6 Ribosomal Protein (phospho Ser235/236) Signal transduct h.m.rt Rabbit-mAb 55
EGFR (E114) Epithelial differentiation h.m.rt Rabbit-mAb 56
p38 MAPK (phospho Thr180/Tyr182) Signal transduct h.m.rt Rabbit-mAb 57
Akt (phospho Ser473) Signal transduct h.m.rt Rabbit-mAb 58
Peanut Agglutinin (PNA-Biotinylated) * - - Lectin 59
CD68 Macrophage m,rt Rabbit-p 60
SMA (alpha) (1A4) Smooth Muscle Actin rb Ms-mAb 61
PCNA Proliferation h.m.rt Rabbit-p 62
TTF-1 (EPR5955(2) Differentiation lung-cancer h.m.rt Rabbit-mAb 63
Fas L Tumor necrosis factor family h.m.rt Rabbit-p 65
Ym1 Macrophage m Goat-p 66
CD24 B-cells h.m.rt Rabbit-p 68
Akt1 (C73H10) Signal transduct h.m.rt Rabbit-mAb 70
Stat3a Signal transduct h.m.rt Rabbit-mAb 71
Stat3 (phospho Tyr705) Signal transduct h.m.rt Rabbit-mAb 72
IgG (HRP) Antibody isotype m Goat-p 73
RFP Red fluorescent protein RFP Rabbit-p 75
ATP1a3 Na/K ATPase m.rt Rabbit-p 78
proSP-C Type II alveolar epithelial cells h.m.rt Rabbit-p 79
CD8a (Fronze only) T cell m Rat-mAb 81
Brdu Proliferation - Sheep-p 82
Pax-7 Myogenesis h.m.rt Rabbit-p 83
HIF-1a Endogenous hypoxia markers h.m Rabbit-p 84
Cadherin, N- Metastatic carcinoma unknown primary m Rat-mAb 85
Cytokeratin 19 Epithelial tumor marker m Rat-mAb 87
EpCAM (G8.8) Carcinoma marker m Rat-mAb 88
Brdu Proliferation - Ms-mAb 90
GFP Green fluorescent protein - Ms-mAb 91
Insulin Pancreatic endocrine tumors h.m.rt Guinea Pig-p 94
Collagen I Fibrillar-forming-collagen h.m Goat-p 95
Ly-6G (1A8) Granulocytes m Rat-mAb 96
Gr-1 (Biotin) (RB6-8C5)(Fronze only) Monocyte, neutrophils and eosinophils (granulocytes) m Rat-mAb 97
Lam-a1 Basement membrane h.m Rabbit-p 98
Laminin Basement membrane h.m.rt Chicken-p 99
Galactosidase (Beta-gal) (IF) Hydrolase enzyme - Chicken-p 100
Transferrin Erythoid marker h.m.rt Rabbit -p 102
GFP (Biotin) Green fluorescent protein - Goat-p 103
c-Myc (Y69) Proto-oncogene/Proliferation h.m.rt Rabbit-mAb 104
LC3A (D50G8) Autophagosome Marker h.m.rt Rabbit-mAb 107
CD3 (SP7) T cell h.m.rt Rabbit-mAb 108
CD43 (S7) Cell adhesion and T-cell activation m Rat-mAb 109
CD44 (IM7) Cell adhesion and migration m Rat-mAb 110
TER-119/Erythroid Cells Erythroid Cell Marker m Rat-mAb 111
Ki67 (B56) Proliferation h.m.rt.p Ms-mAb 112
CD11c (HL3)(Fronze only) Dendritic cells m. Hamster-mAb 113
VEGF Misc tumors h.m.rt Pabbit-p 114
Smad2 (phospho Ser465/467) Signal transduct h.m.rt Rabbit-p 117
CD68 (KP1) Macrophage h Ms-mAb 122
CDk-2 Cell cycle marker h.m.rt Rabbit-p 125
EMBP Eosinophil marker h.m.rt Goat-p 126
Hypophosphorylated Neurofilament H (NF-200) Neurodegeneration marker h.m.r Ms-mAb 128
Tyrosine Hydroxylase Dopaminergic neuron marker h.m Rabbit-p 129
SMA Ab-1 (alpha) (1A4) (Biotin) Smooth Muscle Actin h.m.rt,rb Ms-mAb 130
FSP1 Fibroblast marker h.m Rabbit-p 131
PDGFæR alpha Platelet Derived Growth Factor Recept m Goat-p 132
GFP Green fluorescent protein - Chichen IgY 133
CD11c (N418)(Fronze only) Dendritic cells h.m Hamster-mAb 135
p53(FL-393) tumor suppressor gene h.m.r Rabbit-p 137
E-Cadherin (36/E-Cadherin) Cell-cell interaction, Differentiation h.m.r Ms-mAb 140
N-Cadherin (32/N-Cadherin) Metastatic carcinoma unknown primary h.m.r Ms-mAb 141
Cyclin D1 / Bcl-1 Antibody (SP4) Proliferation h.m.r Rabbit-mAb 142
CD45 Leukocyte differentiation h.m.r.p Rabbit-p 143
CD20 B-cell h Rabbit-mAb 144
PAX5 Transcription Factor (B cell) h.m.r Rabbit-p 145
Cyclin B1 AB-2 Proliferation h.m.r Rabbit-p 146
CD31 Zinc-fixation Endothelial cells m. Rat-mAb 147
CD8 Zinc-fixation T cell h.m.rt Rabbit-mAb 148
Beta-Tubulin (9F3) Neuronal Marker h.m.rt Rabbit-mAb 149
Cyclin A AB-7 Proliferation h.m.r Rabbit-p 150
FOXP3 Transcription regulator of T Cells h.m.r Rabbit-p 151
Fra-2 (H-103) Transcription Factor h.m.r Rabbit-p 152
CD56 (RNL-1) Neuroendocrine differentiation h.m.r Ms-mAb 155
Caspase-3 (3CSP03) Apoptosis h.rb Ms-mAb 156
Beta III Tubulin Neuronal Marker h.m.r.p Rabbit-p 157
VCAM1[EPR5047] Activated Endothelial Cell h.m.rt Rabbit-mAb 158
