The mission of the Northwestern University Mouse Histology & Phenotyping Laboratory (MHPL) is to assist primary investigators and researchers with standard and customized research-specific histology services. The laboratory provides comprehensive histology services for a wide variety of tissues (i.e., mouse, rat, sheep, pig, zebrafish, etc.) for routine histology, and immunohistochemistry. Pathologist consultation can also be provided to help develop strategies to elucidate phenotypes and gain mechanistic insight regarding the biologic actions of the targeted molecule(s) or the toxicity of exogenously administered substances. In addition, the laboratory provides training opportunities for learning histology techniques and phenotyping analysis.
- Immunohistochemistry (IHC) – Using tyramide signal amplification (TSA) and ABC methods (automated), with DAB chromogenic substrate. See documents below for details.
- Immunofluorescence (IF)- Single and multiple staining
- TUNEL assay
- Freezing and embedding of tissues for histology
- Frozen sections
- Paraffin block processing and Paraffin sections
- Hematoxylin and Eosin staining of sections
- Specialized histochemical staining of sections (e.g. Trichrome, PAS, Luxol fast blue, Cresyl violet, and many other stains)
- Dissection and tissue collection
- Histopathology (slide interpretation)
- Histopathology toxicity evaluation
- Training in necropsy techniques including perfusion of mice and special tissue dissection
- Assistance with development of animal pathology protocols